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Polymerase Chain Reaction (PCR): Principle, Types, Procedure, Applications, Advantages, Limitations & Latest Advances (2026 Guide)

  Introduction Imagine being able to create millions of copies of a single DNA fragment within just a few hours. This remarkable capability has revolutionized modern biology, medicine, agriculture, forensic science , and biotechnology. The technology responsible for this breakthrough is the Polymerase Chain Reaction (PCR) , one of the most influential molecular biology techniques ever developed. PCR is a laboratory method used to amplify a specific DNA sequence, producing millions to billions of identical copies from a minute amount of starting DNA. Before the invention of PCR, scientists required relatively large quantities of DNA for genetic analysis, making many experiments time-consuming and technically challenging. Today, PCR allows researchers to analyze DNA from tiny biological samples such as a drop of blood, a strand of hair, a saliva swab, plant tissue, microbial cultures, or even ancient archaeological specimens. Since its invention in 1983, PCR has transformed scientifi...